Pathway Enrichment
About This Analysis
Pathway enrichment tests whether genes in each cluster are over-represented in curated ASD-related biological pathways. Statistical significance is assessed using the hypergeometric test with Benjamini-Hochberg FDR correction. Note: Due to the modest sample sizes (241 genes across 12 clusters), few enrichments reach strict FDR significance. The patterns shown below suggest biological trends that would benefit from validation in larger datasets.
Pathways Tested
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Curated gene sets
Enriched (p < 0.05)
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Before FDR correction
Best Fold Enrichment
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Best p-value
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Pathway Enrichment Dot Plot
Dot size = number of overlapping genes | Color intensity = -log10(p-value) |
Hover for details
What you're seeing: Each dot shows the enrichment of a pathway (columns) within a cluster (rows).
Larger dots indicate more genes overlap; green dots indicate statistically significant enrichment (p < 0.05).
What it means: Clusters with significant pathway enrichment suggest those genes may share biological functions related to that pathway.
What it means: Clusters with significant pathway enrichment suggest those genes may share biological functions related to that pathway.
p < 0.05
p < 0.10
p >= 0.10
Cluster-Pathway Relationships
Flow from clusters to enriched pathways (showing associations with fold enrichment > 1)
What you're seeing: A Sankey diagram showing connections between gene clusters (left) and biological pathways (right).
Flow width represents the number of overlapping genes.
What it means: Clusters connected to multiple pathways may represent genes involved in related biological processes. Green pathways have significant enrichment (p < 0.05).
What it means: Clusters connected to multiple pathways may represent genes involved in related biological processes. Green pathways have significant enrichment (p < 0.05).
Gene-Pathway Network
Genes connected through shared pathway membership (drag to explore, scroll to zoom)
What you're seeing: A network where genes are nodes and edges connect genes that share pathway membership.
Node size reflects the number of pathways a gene belongs to; node color indicates the primary pathway.
What it means: Highly connected genes participate in multiple pathways and may represent key regulatory nodes. Use the dropdown to focus on a specific pathway.
What it means: Highly connected genes participate in multiple pathways and may represent key regulatory nodes. Use the dropdown to focus on a specific pathway.
All Enrichment Results
What you're seeing: All pathway enrichment tests showing overlap count, fold enrichment, and p-value for each cluster-pathway pair.
What it means: Fold enrichment > 1 indicates more genes than expected by chance. Lower p-values (especially < 0.05) suggest statistically significant pathway involvement.
What it means: Fold enrichment > 1 indicates more genes than expected by chance. Lower p-values (especially < 0.05) suggest statistically significant pathway involvement.
| Cluster | Pathway | Overlap | Fold Enrichment | p-value | Overlapping Genes |
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